Probit Analysis for Determining Bacteriophage Lethal Time [LT50] Against E. coli

Amr, Amara A.; Soheir, Salem R.

doi:10.21608/jhiph.2006.158865

Probit Analysis for Determining Bacteriophage Lethal Time [LT50] Against E. coli

Document Type : Original Article

Authors

Amara A. Amr ¹
Salem R. Soheir ²

¹ Genetic Engineering and Biotechnology Research Institute [GEBRI], Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt

² Faculty of Education, Alexandria University, Egypt

10.21608/jhiph.2006.158865

Abstract

Bacteriophage could play a major role in controlling pathogenic bacteria especially those resistant to antibiotics. One of the most important problems in phage techniques that there are no efficient methods for determination of the time required by particular phages number to destroy specific bacterial population during their normal growth activity in a particular sample. In vivo usage of phage needs more accurate methods for determining the phage amount which could be used, where extra dosage could stimulate the patient immune system to produce phage-neutralizing antibodies as well as using plating method [standard double-ager-layer] did not represent what happens in vivo or in fluid samples contaminated with bacteria such as blood or food liquid stuff. The study aimed at analyzing the phage LT₅₀ considering phage and E. coli used in this study as a model. The decrease of the colony forming unit [CFU] after adding different phage concentrations to E. coli culture has been calculated against the time comparing with those in untreated control using Probit analysis method. It is recommended using the study as a standard method for determining the effective dosage of phage that could be used in controlling bacteria growing in different environmental conditions at certain time period.

Keywords