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Hassan, A., El-Sherbini, E., El-Sawaf, G., Hamdy, H., El-Kholy, Z. (2005). Biochemical View on the Effect of Hepatitis Delta Virus Infection on Expression of Hepatitis B Virus in Blood Donors. Journal of High Institute of Public Health, 35(1), 127-142. doi: 10.21608/jhiph.2005.180236
Ashraf A. Hassan; Eglal El-Sherbini; Gamal El-Sawaf; Hanan Hamdy; Zienat A. El-Kholy. "Biochemical View on the Effect of Hepatitis Delta Virus Infection on Expression of Hepatitis B Virus in Blood Donors". Journal of High Institute of Public Health, 35, 1, 2005, 127-142. doi: 10.21608/jhiph.2005.180236
Hassan, A., El-Sherbini, E., El-Sawaf, G., Hamdy, H., El-Kholy, Z. (2005). 'Biochemical View on the Effect of Hepatitis Delta Virus Infection on Expression of Hepatitis B Virus in Blood Donors', Journal of High Institute of Public Health, 35(1), pp. 127-142. doi: 10.21608/jhiph.2005.180236
Hassan, A., El-Sherbini, E., El-Sawaf, G., Hamdy, H., El-Kholy, Z. Biochemical View on the Effect of Hepatitis Delta Virus Infection on Expression of Hepatitis B Virus in Blood Donors. Journal of High Institute of Public Health, 2005; 35(1): 127-142. doi: 10.21608/jhiph.2005.180236

Biochemical View on the Effect of Hepatitis Delta Virus Infection on Expression of Hepatitis B Virus in Blood Donors

Article 10, Volume 35, Issue 1, January 2005, Page 127-142  XML
Document Type: Original Article
DOI: 10.21608/jhiph.2005.180236
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Authors
Ashraf A. Hassan1; Eglal El-Sherbini2; Gamal El-Sawaf2; Hanan Hamdy3; Zienat A. El-Kholy3
1Applied Medical Chemistry Department, Medical Research Institute, Alexandria University, Egypt
2Microbiology Department, Medical Research Institute, Alexandria University, Egypt
3Applied Medical chemistry Department, Medical Research Institute, Alexandria University, Egypt
Abstract
One thousand voluntary blood donors attending the Blood Bank of Medical Research Institute, Alexandria University were enrolled in the present study. Sera from blood samples were separated for screening of HBsAg using ELISA technique. Screening of 200 HBsAg negative blood samples for past exposure to HBV infection was also performed through the detection of anti-HBc using specific ELISA kit. Determination of ALT and AST revealed a significant increase among HBsAg positive group while, Malondialdehyde [MDA] detection revealed the absence of the causative relationship between MDA and liver injury. On the other hand, no anti-HDV could be found among the HBsAg –ve/anti-HBc +ve group of blood donors [46 cases]. PCR analysis for HBV-DNA was performed in the chosen 24 serum samples. None of them was positive for HBV-DNA regardless their positively or negativity to the different serologic markers studied.   
Keywords
Hepatitis Delta Virus Infection; Hepatitis B Virus; Blood Donors
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