Effect of Aspartame and Sucralose Artificial Sweeteners on Weight and Lipid Profile of Male Albino Rats

Khamise, Nermin A.; Tayel, Dalia I.; Helmy, Maged W.; Aborhyem, Samar

doi:10.21608/jhiph.2020.108281

Effect of Aspartame and Sucralose Artificial Sweeteners on Weight and Lipid Profile of Male Albino Rats

Document Type : Original Article

Authors

¹ Faculty of Allied Medicine, Pharos University, Alexandria, Egypt

² Department of Nutrition, High Institute of Public Health, Alexandria University, Egypt

³ Department of Pharmacology and Toxicology, Faculty of Pharmacy, Damanhur University, Egypt

⁴ Food Analysis Unit, Department of Nutrition, High Institute of Public Health, Alexandria University, Egypt

10.21608/jhiph.2020.108281

Abstract

Background: Artificial sweeteners interfere with normal physiological processes.
Objective: The study aims at assessing the changes associated with consuming different doses of aspartame (Sugar-Match^®) and sucralose (Sweetal^®).
Methods: A total of sixty rats were divided into two phases; phase I was categorized into 6 groups including a control group, sucralose 2 and 4 g/kg, aspartame 0.8 and 1.6 g/kg, and sucrose with dose 0.5 mg/kg given orally every day for 12 weeks. Rats were euthanized and lipid profile was measured. Phase II comprised 4 groups including the same previously mentioned doses of sucralose and aspartame which were given orally every day for 12 weeks then omitted for further 6 weeks to study the ability of body to restore the biological changes associated with their consumption.
Results: The highest triglyceride level was observed in rats fed on high dose sucralose (80.83 ± 5.46 mg/dl) and aspartame (78.83 ± 4.17 mg/dl). After 12 weeks of experimentation, cholesterol was higher in all groups. LDL-C was the highest in rats supplemented with a high dose of aspartame (43.90 ± 8.41 mg/dl), followed by a low dose of aspartame (39.28 ± 2.03 mg/dl). Terminating intake of artificial sweeteners caused large drop in LDL-C in rats fed on high dose of aspartame, while HDL-C increased slightly but insignificantly. Severe histopathological changes in liver and kidney tissues were observed in rats supplemented with a high dose of aspartame.
Conclusion: Supplementing rats with aspartame and sucralose for 12 weeks increased lipid profile. Pathological changes were recovered neither in the liver nor in the kidney even after terminating artificial sweeteners intake.

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